Biological Buffers
Thermo Scientific™ 6X DNA Loading Dye
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature
Invitrogen™ Potassium Acetate (3M), pH 5.5
Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature
GE Healthcare Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 3 - 10Store in Fridge at 4C
Invitrogen™ EDTA (0.5M), pH 8.0
Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature
Invitrogen™ Tris (1M), pH 8.0
Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature
Laemmli SDS Sample Buffer, reducing, 6X
For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, reducing (6X) 50ml
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TS SDS 10X RUN BUF, 5L
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100GR HEPES (Fine White Crystals) for Molecular Biology,
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL
Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER
Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents
Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology
Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL
Invitrogen™ BSA, acetylated (20 mg/mL)
Stabilizes enzymatic reactions to ensure the absence of contaminutesants ACETYLATED BSA (20MG/ML)6X25MGAmbion® Acetylated BSA is used as a carrier
Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is
Thermo Scientific™ Blocker™ BSA Blocking Buffers
Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML
Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X
Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C
Alfa Aesar™ HEPES hemisodium salt
CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 500GR HEPES hemisodium salt
Thermo Scientific™ MgCl2 (Magnesium Chloride)
Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2
Invitrogen™ Sodium Acetate (3M), pH 5.5
Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature
Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)
LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C
Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT
Invitrogen™ Proteinase K
Remains active under various conditions 1 GR PROTEINASE K STORE AT 2° TO 8°C
Lonza™ Ficoll™ DNA Loading Buffer, 6X
Ideal for improving resolution and separation of DNA fragments DNA Loading Buffer 6X FP=5 x 1 ml
MES, 99+%, <1% water, ACROS Organics™
CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00006181 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O 50GR MES, 99%, <1% water
Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)
Invitrogen™ Glycogen (5 mg/ml)
Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate