Thermo Scientific Molecular Biology Solutions

Molecular Cloning

Molecular cloning produces identical, recombinant DNA molecules for downstream applications, such as gene expression, protein purification and mutational analysis. Genes of interest are cloned into plasmid vectors, which are transformed into bacterial cells to produce bacterial clones. Thermo Scientific offers the most reliable genetic content, superior enzymes and advanced PCR tools to support and streamline your entire cloning workflow.

Find out more about Thermo Scientific cloning products starting from sample preparation to fragment amplification, DNA modification, electrophoresis and ligation.

• Cloning Solutions (PDF 4.25MB)
• aLICator LIC Cloning and Expression System (PDF 2.76MB)

PCR & qPCR

PCR is used to detect the presence or absence of specific DNA sequences, amplify large amounts of DNA for downstream use, or mutagenize coding DNA, for further genetic manipulation, mutational analysis, sequencing, genotyping or cloning. Depending on the polymerase used, amplification can be highly robust, quick or error proof, as in the case of high-fidelity enzymes. If starting material is a concern, direct PCR can be used to eliminate sample preparation altogether, or Reverse Transcription-PCR can be used if starting with mRNA. Quantitative real time PCR (qPCR) allows for real-time results with less variability due to sensitive fluorescent chemistry and elimination of post-PCR detection procedures.

Thermo Scientific offers PCR/qPCR solutions for your workflows; choose a polymerase or an appropriate PCR method for your needs using the following chart. Choose the best enzyme for your application or choose your detection chemistry with the PCR/qPCR Solutions brochure.

• PCR Reagents Selection Guide (PDF 102KB)
• PCR/qPCR Solutions (PDF 6.22MB)