Antibotics, Buffers and Supplements

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Corning™ Sodium Pyruvate 100mM Solution

Suggested replacement for 25-000-CIR

Merck Millipore GS System 100mM L-Methionine sulfoximine

utilizes specific vectors for cell culture transfection.

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Corning™ ITS+ Premix Universal Culture Supplement

Stimulates proliferation of a variety of cells under serum-reduced conditions. Corning™ ITS+ Premix Universal Culture Supplement contains insulin, human transferrin and selenous acid, the three most universally esential components of defined culture media.

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing

Gibco™ Polypeptone™ Peptone

Pancreatic digest of casein and peptic digest of animal tissue combined in equal parts

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Merck Millipore Chemicon™ ESGRO™ Leukemia Inhibitory Factor (LIF), 10 million units/1mL

Optimized formulation of recombinant mouse LIF (mLIF) for in vitro maintenance of mouse ES and iPS cells.

Corning™ Accutase™ Cell Detachment Solution

For detachment of adherent cells, primary stem cell lines and tissues

MP Biomedicals™ Poly-L-Lysine Hydrobromide Attachment Factors: ≥70,000 MW

Facilitate attachment, adherence, and spreading in the culture of adherent cells

MES sodium salt, 99%, ACROS Organics™

CAS: 71119-23-8 Molecular Formula: C6H12NNaO4S Molecular Weight (g/mol): 217.215 MDL Number: MFCD00065473 InChI Key: IRHWMYKYLWNHTL-UHFFFAOYSA-M PubChem CID: 23673676 ChEBI: CHEBI:62955 IUPAC Name: sodium;2-morpholin-4-ylethanesulfonate SMILES: C1COCCN1CCS(=O)(=O)[O-].[Na+]

Gibco™ Bacto™ Tryptone

For use with dehydrated media culture

Corning™ Fibronectin, Human

Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media.

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Corning™ 2.5% Trypsin in HBSS w/o Calcium, Magnesium and Phenol Red

Enzymatic dissociation reagents tested for porcine parvovirus

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Corning™ Trace Elements

For Research applications

HEPES, >98%, MP Biomedicals™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N?-(2-ethanesulfonic acid) sodium salt PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Corning™ Selenous Acid (Sodium Salt)

Shown to promote proliferation of MDCK cells in serum-free medium

HEPES, 99%, for biochemistry, ACROS Organics™

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

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