IBA GmbH
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Twin-Strep-tag™/Strep-Tactin™XT
The purification system with the highest affinity – one tag for expression, purification, detection and immobilization
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Immobilization with near covalent binding affinity
Twin-Strep-tag™ Capture Kit for SPR experiments »
- High affinity in low pM range enables long term measurements (T1/2 = 13 h)
- Chip can be easily regenerated
High affinity meets high capacity!
Strep-Tactin™XT Superflow™ high capacity »
- Higher protein yields compared to all other Strep-Tactin™ resins
- Dynamic binding capacity of ~15 mg protein per ml resin
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
Try our new high affinity Strep-tag™ system
Starter Kit consists of:
- Strep-Tactin™XT Superflow™ gravity flow column
- Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
- Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
- Strep-Tactin™ conjugated with HRP (for Western blot detection)
- Short protocol
Traceless Affinity Cell Selection
Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection
- High purity, yield and viability
- Label-free cells
- Directly from whole blood
- No centrifugation
Magnetic Cell Selection
- Fully reversible
- No stimulation of cells after cell separation
- Optimal preservation of cell effector function
- Improved viability of cells
Cell Stimulation & Expansion
- Completely reversible reagents
- Detachment from the cells at any given point in time
- Adjustable CD3 : CD28 ratio
- Non-magnetic and bead-free
Peptides for T cell stimulation »
- Purity > 80 %
- Endotoxin free
Fluorescent Cell Staining
Detection of antigen-specific T cells with MHC I Streptamers™ »
Detection of receptor-specific T cells with Fab Streptamers™ »
- Fully reversible
- High specificity, sensitivity and reproducibility
- No leftover of staining reagents on the cells after FACS sorting
- Optimal preservation of cell effector function
- Improved viability of cells
Cloning & Transfection
More than 150 expression vectors »
- Variety of affinity tags
- Expression hosts: E. coli, mammalia, insect cells, yeast
- StarGate cloning system
Mammalian Expression
Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.
Protein Purification
Strep-tag™ system from the original manufacturer
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Detection
For Western blot analysis, immunofluorescence, FACS and ELISA »
- Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
- Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™
Immobilisation
Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)
- High affinity in low pM range enables measurements over a long time
- Minimal non-specific binding
- Minimal coefficients of variation (cv)
- Chip for SPR experiments can be easily regenerated
Immunoprecipitation
Selector resins based on high-affinity single-domain antibodies (sdAb)
- High capacity
- Minimal unspecific binding
DNA
RNA
Standard DNA oligonucleotides
- Long range up to 200 bases*
- Large scale oligonucleotides
- 3’-, 5’- or internally modified
Random RNA, dsRNA, siRNA, miRNA
- Long range up to 100 bases*
- 3’-, 5’- or internally modified
- 2‘-modified: -O-Me, -F, -NH2
- Nucleoside Triphosphates
- RNA and RNA/DNA hybrid
- Dinucleotides
*depending on sequence and scale
Fluorescence Labels
Non-Fluorescence Labels
E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami
- For dual and multiply labeled DNA and RNA
- Excellent high quality
- More than 200 labels to choose from
- Quenchers such as BBQ and BHQ™
- Completely new dye combinations available
For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more
- More than 80 internal, 5’- and 3’- DNA and RNA modifications
Backbone Modifications
Aptamers
- Phosphorothioates
Custom synthesis of DNA and RNA aptamers
- High affinity
- Low immunogenicity
Cell Selection & Expansion
- Application Note FABian Lymphocyte Isolation
- Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
- Application Note Combining MHC-I and CD8+ Fab Streptamer reagents
- Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
- Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Custom Oligos & Predefined DNA/RNA
About IBA GmbH
Solutions For Life Sciences
IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.