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Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.
Brand: Thermo Scientific™ F140WH
This item is not returnable.
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Description
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds, and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, dilution buffer, and DNARelease additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species.
Features
- No need for time-consuming and expensive DNA purification steps
- Very little sample material required
- Two simple protocols for various applications
- Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).
Notes
- A detailed manual is provided that describes protocols when working with different types of tissues.
- The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
Specifications
| • Phire Hot Start II DNA Polymerase, 200 μL • 2X Phire Animal Tissue PCR Buffer, 5 x 1.25 mL • Control Primer Mix (25 μM each), 40 μL • Dilution Buffer, 2 x 5 mL • DNARelease Additive, 3 x 100 μL • Gel Loading Dye, 3 x 1 mL Store at -20°C. |
|
| Direct PCR | |
| Fast | |
| Wet or Dry Ice | |
| 500 reactions | |
| Separate Components | |
| DNA |
| Medium | |
| Phire Hot Start II DNA Polymerase | |
| Direct PCR Kit | |
| Built-In Hot Start | |
| Blunt | |
| Tissue |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Phire Animal Tissue Direct PCR Kit/DNARelease Additive
Signal Word
- Danger
Hazard Category
- Respiratory sensitiser Category 1
Hazard Statement
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.