Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Brand: Thermo Scientific™ 34095
337.35 EUR valid until 2025-03-21
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Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.
SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.Highlights:
ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes
Quantitative – produces quantitative signal that is measurable over two orders of magnitude
Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)
Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system
Excellent signal duration – 8 hours of usable light-output when conditions are optimized
Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 100 mL | |
| 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL) | |
| 6 to 8 hr. | |
| Luminol/Enhancer (50 mL), Stable Peroxide Buffer (50 mL) |
| Chemiluminescence | |
| Western Blot | |
| SuperSignal™ | |
| Horseradish Peroxidase | |
| Low femtogram-level | |
| 12 Mini-Gel Blots, 1000 sq. cm of Membrane | |
| Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane • Luminol/Enhancer, 50 mL • Stable Peroxide Buffer, 50 mL Store reagents at 4°C. |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| ZL400249 | Certificate of Analysis | 31/01/2025 | 34095_LITL18 PROMO,34095 |
| AA402134A | Certificate of Analysis | 31/01/2025 | 34095_LITL18 PROMO,34095 |
| AA402134 | Certificate of Analysis | 31/01/2025 | 34095_LITL18 PROMO,34095 |
| ZJ399323 | Certificate of Analysis | 16/12/2024 | 34095_LITL18 PROMO,34095 |
| ZJ399323A | Certificate of Analysis | 16/12/2024 | 34095_LITL18 PROMO,34095 |
Expires: 2023-06-09
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For Research Use Only. Not for use in diagnostic procedures.
