Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Brand: Thermo Scientific™ 34096
588.02 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.
SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.Highlights:
ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes
Quantitative – produces quantitative signal that is measurable over two orders of magnitude
Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)
Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system
Excellent signal duration – 8 hours of usable light-output when conditions are optimized
Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 200 mL | |
| Horseradish Peroxidase | |
| Low femtogram-level | |
| 25 mini-gel blots, 2000 sq. cm of membrane | |
| Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane • Luminol/Enhancer, 100 mL • Stable Peroxide Buffer, 100 mL Store reagents at 4°C. |
| Chemiluminescence | |
| Western Blot | |
| SuperSignal™ | |
| HRP | |
| 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL) | |
| 6 to 8 hr. | |
| Stable Peroxide Buffer (100 mL), Luminol/Enhancer (100 mL) |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| ZL410861 | Certificate of Analysis | 31/01/2025 | 34096_LITL18 PROMO,34096 |
| ZL400250 | Certificate of Analysis | 31/01/2025 | 34096_LITL18 PROMO,34096 |
| ZL400250A | Certificate of Analysis | 31/12/2024 | 34096_LITL18 PROMO,34096 |
| ZJ399324 | Certificate of Analysis | 16/12/2024 | 34096_LITL18 PROMO,34096 |
| ZJ397736 | Certificate of Analysis | 06/11/2024 | 34096_LITL18 PROMO,34096 |
Expires: 2022-02-28
*Discount will apply to qualifying orders received by Fisher Scientific between 01/01/2022 and 28/02/2022. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.
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For Research Use Only. Not for use in diagnostic procedures.
