IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin

Description

• Strep-Tactin™XT 4Flow™ high capacity is a 4% agarose coupled with the streptavidin variant Strep-Tactin™XT and applicable for purification of Strep-tag™II or Twin-Strep-tag™ fusion proteins.
• Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommend for all protein classes, especially for low abundant or challenging proteins.
• The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets.
• In addition, Strep-Tactin™XT 4Flow™ high capacity allows the application of denaturing agents such as urea.
• In comparison to Strep-Tactin™XT 4Flow™, Strep-Tactin™XT 4Flow™ high capacity has a higher density of immobilized Strep-Tactin™XT, which leads to a 2-fold higher yield of purified protein.
• The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification.
• To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.