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IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

266.00 EUR - 286.00 EUR

Specifications

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
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Products 8
Product Code Brand Protein Tag Vector Price Quantity & Availability  
Product Code Brand Protein Tag Vector Price Quantity & Availability  
15603888
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IBA Lifesciences
5-5214-001
His Tag (6x), Strep-tag II pDSG-IBA45
266.00 EUR
5µg
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15613888
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IBA Lifesciences
5-5219-001
Twin-Strep-tag pDSG-IBA102
286.00 EUR
5µg
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15623888
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IBA Lifesciences
5-5220-001
Twin-Strep-tag pDSG-IBA103
286.00 EUR
5µg
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15633888
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IBA Lifesciences
5-5221-001
Twin-Strep-tag pDSG-IBA104
286.00 EUR
5µg
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15643888
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IBA Lifesciences
5-5222-001
Twin-Strep-tag pDSG-IBA105
286.00 EUR
5µg
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15663878
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IBA Lifesciences
5-5200-001
Untagged pDSG-IBAwt1
286.00 EUR
5µg
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15673878
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IBA Lifesciences
5-5201-001
Untagged pDSG-IBAwt2
286.00 EUR
5µg
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15693878
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IBA Lifesciences
5-5211-001
His Tag (6x), Strep-tag II pDSG-IBA43
266.00 EUR
5µg
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Description

Description

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Specifications

Constitutive
CMV
Geneticin™ (G-418)
Mammalian
250 ng/μL
Ampicillin
Vector
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Direct Cloning
5 μg
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