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IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
266.00 EUR - 286.00 EUR
Specifications
Constitutive or Inducible System | Constitutive |
---|---|
Marker Gene | Ampicillin |
Promoter | CMV |
Product Type | Vector |
Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
Product Code | Brand | Protein Tag | Vector | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Protein Tag | Vector | Price | Quantity & Availability | ||||
15603888
|
IBA Lifesciences
5-5214-001 |
His Tag (6x), Strep-tag II | pDSG-IBA45 |
266.00 EUR
5µg |
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15613888
|
IBA Lifesciences
5-5219-001 |
Twin-Strep-tag | pDSG-IBA102 |
286.00 EUR
5µg |
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15623888
|
IBA Lifesciences
5-5220-001 |
Twin-Strep-tag | pDSG-IBA103 |
286.00 EUR
5µg |
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15633888
|
IBA Lifesciences
5-5221-001 |
Twin-Strep-tag | pDSG-IBA104 |
286.00 EUR
5µg |
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15643888
|
IBA Lifesciences
5-5222-001 |
Twin-Strep-tag | pDSG-IBA105 |
286.00 EUR
5µg |
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15663878
|
IBA Lifesciences
5-5200-001 |
Untagged | pDSG-IBAwt1 |
286.00 EUR
5µg |
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15673878
|
IBA Lifesciences
5-5201-001 |
Untagged | pDSG-IBAwt2 |
286.00 EUR
5µg |
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15693878
|
IBA Lifesciences
5-5211-001 |
His Tag (6x), Strep-tag II | pDSG-IBA43 |
266.00 EUR
5µg |
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Description
pDSG-IBA vectors for MEXi mammalian expression system
- Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
- Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
- Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number
Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Specifications
Constitutive | |
CMV | |
Geneticin™ (G-418) | |
Mammalian | |
250 ng/μL |
Ampicillin | |
Vector | |
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
Direct Cloning | |
5 μg |