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Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification
Best choice for cost effective GST-tag protein purification.
Brand: Macherey-Nagel™ 745500.100
Alert:
Do not autoclave the gel.
Description
Protino™ Glutathione Agarose 4B is an affinity chromatography medium that enables fast, single-step purification of glutathione-S-transferase (GST) fusion proteins and other glutathione-binding proteins. Purification of fusion proteins from a whole cell lysate is based on the strong affinity of the GST moiety for glutathione, which is immobilized on agarose beads.
- Highest performance–Equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
- Simply replace your current products—No optimization or protocol change necessary
- Universal usage—Suitable for small proteins, large protein complexes, proteins with low expression rates universal use
- Highest flexibility of applications—For batch binding, gravity-flow column chromatography, MPLC/FPLC™
Specifications
| 100 mL | |
| 0.1 M acetate, pH 4, 0.1 M NaOH, 70% ethanol, or 6 M guanidine hydrochloride | |
| Settled beaded agarose | |
| 4°C to 8°C for up to 3 years | |
| 100 mL | |
| Agarose | |
| Chelating ligand: Glutathione, linked via sulfur atom |
| 8mg/mL | |
| 250 cm/hr. | |
| 4% beaded agarose | |
| 90 ÎĽm | |
| Purification of GST-tagged proteins using affinity chromatography | |
| 0.2 to 1.0 mL/min. (with 1 mL bed volume/column with 6.6 mm I.D.), 0.5 to 5.0 mL/min. (with 10 mL bed volume/column with 16 mm I.D.) |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Protino Glutathione Agarose 4B
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Supplemental information
- MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU)
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Product Title
Macherey-Nagel™ Protino Glutathione Agarose 4B for GST-tag Protein Purification > Quantity: 100 mL
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