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  7. Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
  8. 10229194

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Brand:  Applied Biosystems™ 4389986

489.50 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10229194

  • 890.00 EUR / 200 reactions

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Description

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

  • Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
  • Formulated to ensure maximum sensitivity and reliability
  • Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

Order Info

Shipping Conditions: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Sufficient for 200 reactions at 50 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
1-step RT-qPCR
Standard
SYBR Green
RNA-to-CT™, SYBR™
UP (Ultra Pure)
ArrayScript™ UP
Wet Ice
Gene Expression
RNA
SYBR
AmpliTaq Gold DNA Polymerase
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
ROX (Pre-mixed)
One-Step qRT-PCR Kit
200 reactions
Standard
200 Reactions
200 Reactions
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For Research Use Only. Not for use in diagnostic procedures.