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Thermo Scientific™ Power-Bind™ Streptavidin Coated Particles
Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles.
Brand: Thermo Scientific™ 29000701011150
This item is not returnable.
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Description
Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances.
The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active formMade from 0.3μm or 0.8μm OptiLink™ Carboxylate-Modified particles
High biotin-binding capacity
Extremely stable, long shelf life
Simplifies the process of binding ligands to particles
Combines the advantages of high surface area and easy, high affinity, high specific activity binding
Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications
Power-Bind Benefits Include:
- Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
- High activity of surface bound ligands can easily solve difficult coupling problems
- Simple aqueous biotinylation reactions
- Low nonspecific interactions (blocked microparticle surface)
- Highly mobile particles for membrane-based applications
- Can be used in EIA formats with biotin/enzyme detection systems
- Beneficial spacer effect of SA molecule
- A choice of 0.3μm or 0.8μm diameters for different application requirements
Recommended for:
Universal nonmagnetic bead for coupling unique ligands. Clinical diagnostics and molecular biology applications.
Specifications
| Includes Certificate of Analysis | |
| Power-Bind Streptavidin Coated Particles | |
| Biotinylated Ligand Binding, Diagnostic Assays | |
| Streptavidin-Coated Particles | |
| 0.05% Na Az | |
| Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate. | |
| Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling |
| 2°C to 8°C | |
| White or Colorless | |
| Polystyrene | |
| Package Insert and Certificate of Analysis | |
| 1 mL | |
| Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request. | |
| 0.3 μm |
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