Thermo Scientific™ Pierce™ Protein L Plus Agarose

Description

Perform a variety of antibody affinity purification methods with Thermo Scientific™ Pierce High-Capacity Protein L Agarose, a high-capacity Protein L beaded agarose resin.

Pierce Protein L Plus Agarose is a high-capacity affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains. Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies. The resin consists of recombinant Protein L immobilized on crosslinked 6% beaded agarose (CL-6B) using a stable, leach-resistant chemistry. The agarose beads can be regenerated and reused multiple times when stored properly.

Highlights:

• Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains

• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods

• Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield

• High capacity – “Plus” variety of Pierce Protein L Agarose has a dense load of immobilized Protein L, providing a binding capacity of 10 to 20mg human IgG/mL resin

Properties of crosslinked 6% beaded agarose (CL-6B):

• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Linear Velocity: 30cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather
• than the pressure drop across the column.)