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Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
Brand: Thermo Scientific™ 21516
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Description
Purify protein interaction complexes involving practically any user-supplied GST-tagged fusion protein with Thermo Scientific™ Pierce GST Protein Interaction Pull-Down Kit.
The GST Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a glutathione S-transferase (GST) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, glutathione affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.Highlights:
GST pull-down – purifies protein interactors of any GST-tagged fusion protein
Complete kit – provides all components and detailed protocol for purifying protein:protein interactions
No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)
Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of glutathione agarose beads, including simple processing of multiple samples
Flexible – instructions include protocols for bait and prey proteins from different sources
Includes:
Glutathione agarose resin, lysis buffer, TBS pack, glutathione for elution, microcentrifuge spin columns and collection tubes
Recommended for:
Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates
Specifications
| Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin • Glutathione Agarose, 750 μL • Pull-Down Lysis Buffer, 250 mL • Glutathione, 1 g • TBS Pack (makes 500 mL), 1 pack • Pierce Spin Columns and Accessories, 27 columns • Microcentrifuge Collection Tubes, 100 tubes Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C. |
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| Microcentrifuge | |
| 25 reactions kit | |
| Not Target-Specific | |
| 25 Reactions |
| Affinity Purification | |
| Pierce™ | |
| Pull-Down (Tag-Based) | |
| Pull-Down Kit | |
| Kit |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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