missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ Pierce™ Glycoprotein Staining Kit
Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.
Brand: Thermo Scientific™ 24562
This item is not returnable.
View return policy
Description
Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.Highlights:
Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band
Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes
Includes:
Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)
Specifications
| Sufficient For: 10 mini gels (SDS-PAGE) • Oxidation Reagent, 2.5 g powder to make 250 mL • Reduction Reagent, 1.25 g powder to make 250 mL • Positive Control (Horseradish Peroxidase), 1 mg • Negative Control (Soybean Trypsin Inhibitor), 1 mg • Glycoprotein Stain, 250 mL Store all components at 4°C. |
|
| Colorimetric | |
| Glycoprotein Staining Kit | |
| 750 mL |
| In-Blot Detection, In-Gel Detection | |
| Fuchsin Sulfite | |
| Pierce™ | |
| Proteins (Glycoproteins) |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Pierce Glycoprotein Staining Kit
Signal Word
- Danger
Hazard Category
- Oxidising solid Category 2
- Acute toxicity Category 4
- Skin corrosion/irritation Category 1 B
- Serious eye damage/eye irritation Category 1
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- Carcinogenicity Category 1B
Hazard Statement
- H272-May intensify fire; oxidiser.
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H350-May cause cancer.
- EUH031-Contact with acids liberates toxic gas.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P220-Keep/Store away from clothing/combustible materials.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P331-Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305-IF IN EYES:.
- P310-Immediately call a POISON CENTER/doctor/.
- P501-Dispose of contents/container to .
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction