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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

123.00 EUR - 1808.00 EUR

Specifications

Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Shipping Condition Dry Ice
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Products 3
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10024537
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Thermo Scientific™
F530S
100 Reactions
123.00 EUR
100 units
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10287787
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Thermo Scientific™
F530L
500 Reactions
530.00 EUR
500 units
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16254551
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Thermo Scientific™
F-530XL
2000 Reactions
1808.00 EUR
2000 units
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Description

Description

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Specifications

Specifications

• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
Fast
Dry Ice
2 U/μL
No
Standalone
High
High-Fidelity DNA Polymerase
Standard PCR, High-fidelity PCR
52X
Blunt
20 kb or less
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SDS
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.