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  7. Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

95.00 EUR - 384.00 EUR

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Specifications

GC-Rich PCR Performance High
Polymerase Phusion Flash II DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Master Mix
Shipping Condition Dry Ice
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10043967
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Thermo Scientific™
F548S 		100 Reactions
95.00 EUR
100 reactions
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10659009
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Thermo Scientific™
F548L 		500 Reactions
384.00 EUR
500 reactions
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Description

Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features

  • Extreme speed with extension times of 15 s/kb or less
  • Hot start modification allows 'zero-time reactivation'
  • Accurate proofreading DNA polymerase with a fidelity of 25X Taq
  • High yields in reduced cycling time
  • Minimized pipetting steps

Applications

  • Fast PCR
  • High-fidelity PCR
  • Difficult (GC-rich) templates
  • Template generation for sequencing Multiplex PCR
  • Long-range PCR
  • Cloning & mutagenesis
  • Microarray

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
Specifications

Specifications

High
Fast
Dry Ice
2X
Built-In Hot Start
SuperMix or Master Mix
Colorless
Phusion Flash II DNA Polymerase
High-Fidelity PCR Master Mix
Hot-start PCR, High-fidelity PCR
25X
Blunt
20 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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