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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 14-5743-82
This item is not returnable.
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Description
Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM. 2. Incubate cells at 37°C for 2 hours. 3. Wash cells with flow staining buffer. 4. Proceed with cell surface staining as normal. For additional information see the references listed below. Purity: Greater than 90%, as determined by SDS-PAGE.
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry.
Specifications
| OVA257-264 (SIINFEKL) peptide bound to H-2Kb | |
| Monoclonal | |
| 0.5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Mouse | |
| 100 μg | |
| Primary | |
| 4° C | |
| Liquid |
| Flow Cytometry, Immunohistochemistry | |
| eBio25-D1.16 (25-D1.16) | |
| Unconjugated | |
| H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb | |
| Affinity chromatography | |
| RUO | |
| Mouse | |
| Antibody | |
| IgG1 κ |
For Research Use Only.