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OPTIZYME™ EcoRI, Fisher BioReagents™

152.00 EUR - 203.00 EUR

Specifications

Components	25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
Incubator Temperature	37°C
pH	7.4
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
Storage Buffer	10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol

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Products 2

Product Code	Brand	Concentration	Quantity	Price	Quantity & Availability
Product Code	Brand	Concentration	Quantity	Price	Quantity & Availability
11924842 View Documents missing translation for 'viewProductCertificates'	Fisher BioReagents BP8003-5	10 U/μL	15,000 U	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

11934812 View Documents missing translation for 'viewProductCertificates'	Fisher BioReagents BP8054-1	50 U/μL	25,000 U	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

Description

5'...G^A A T T C...3'
3'...C T T A A^G...5'

Conditions for 100% Activity:

1X OPTIZYME Buffer EcoRI: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
Incubate at 37°C.

Supplied with: 10X OPTIZYME EcoRI Buffer
Enzyme Activity in OPTIZYME buffers:

Buffer 1: 0 - 20%
Buffer 2: No reaction
Buffer 3: 100%
Buffer 4: No reaction
Buffer 5: 100%™

™star activity occurs at greater than 5-fold overdigestion (5 units X 1 hour)
Storage Buffer:
10mM potassium phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 0.15% Triton X-100 and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with EcoRI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of EcoRI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt concentration, large excess of the enzyme, pH>8.0, or the replacement of mg²⁺ by Mn²⁺ may result in star activity.
Compatible Ends: XapI, MunI, TasI.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: may overlap - cleavage impaired.

5'...m5C GAATTm5C G...3'

3'... Gm5CTTAA Gm5...5'

EcoKI: never overlaps - no effect.

EcoBI: may overlap - no effect.

5'...TGm6AATTC(N)₄TGCT...3'

3'...AC TTAAG(N)₄m6ACGA...5'

Specifications

Components	25 to 50% Water, >50% Glycerin, 2.5 to10% Sodium Chloride
pH	7.4
Storage Buffer	10mM Potassium Phosphate (pH 7.4 at 25°C), 300mM NaCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 0.15% Triton™ X-100, 50% Glycerol
Cut Site	G.AATTC
Form	Liquid

Incubator Temperature	37°C
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with EcoRI
Content And Storage	Keep container tightly closed
Product Type	EcoRI

Safety and Handling

Product Identifier

OPTIZYME

OPTIZYME™ EcoRI, Fisher BioReagents™

Specifications

Description

Specifications

Safety and Handling

Documents