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Invitrogen™ FGF2 Human ELISA Kit

Quantifies human Fibroblast Growth Factor basic (FGF-b) in serum, plasma, buffered solution, or cell culture medium

668.00 EUR - 998.00 EUR

Specifications

Accession Number P09038
Assay Range 15.6-1000 pg/mL
Assay Sensitivity 7 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

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Description

Description

The Human FGF-b ELISA Kit quantifies human Fibroblast Growth Factor basic (FGF-b) in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant FGF basic-146 and FGF basic-157. Principle of the method The Human FGF-b solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Assay recognizes both natural 18 kDa isoform and recombinant FGF basic-146 and FGF basic-157.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

P09038
7 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
BFGF,FGF-2,FGFB,HBGF-2
5.5%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
FGF-Basic
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Human
4 hr.
15.6-1000 pg/mL
Biotin
Plasma, Serum, Supernatant
Colorimetric Detection
2247
4 hr.
4.3%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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