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Invitrogen™ TNFRII (Soluble) Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ KAC1771
Includes: Pre-coated plate, standard, controls, antibody-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Human Tumor Necrosis Factor Receptor II (Hu TNFRII) ELISA quantitates Hu TNFR1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFRII. Principle of the method The Human TNFRII solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Tumor necrosis factor receptor 2 (TNFR2) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating the metabolic effects of TNF-alpha. TNFR2 isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that TNFR2 regulates TNF-alpha function by antagonizing its biological activity.
Specifications
| P20333 | |
| 0.1 ng/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR | |
| 9.2% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls | |
| TNF-RII | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
| 2-142 ng/mL | |
| HRP | |
| Plasma, Serum, Supernatant | |
| Colorimetric Detection | |
| 7133 | |
| 1.25 hr. | |
| 2.9% | |
| HRP | |
| 96 Tests | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| Human | |
| 1 hr. 15 min. |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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