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Invitrogen™ IL-5 Mouse Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of mouse IL-5 protein levels from samples including serum, lavage fluid, and cell culture supernatants.
240.00 EUR - 782.00 EUR
Specifications
| Accession Number | P04401 |
|---|---|
| Assay Range | 4-500 pg/mL |
| Assay Sensitivity | 4 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Alert:
Contains Azide
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
15591017
|
Invitrogen™
88-7054-22 |
2 x 96 Tests |
240.00 EUR
Pack of 2 |
Estimated Shipment: 13-01-2025
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|
15501027
|
Invitrogen™
88-7054-76 |
20 x 96 Tests |
782.00 EUR
Pack of 20 |
Estimated Shipment: 13-01-2025
Log in to see stock. |
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|
15521027
|
Invitrogen™
88-7054-86 |
10 x 96 Tests |
544.00 EUR
Pack of 10 |
In Stock
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Description
The Mouse Interleukin-5 (IL-5) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-5. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Mouse IL-5 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)Specifications
| P04401 | |
| 4 pg/mL | |
| ELISA Kit | |
| Mouse | |
| Colorimetric Microplate Reader | |
| GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF | |
| HRP | |
| Lavage Fluid,100 μL; Serum, 100 μL; Supernatant, 100 μL | |
| Interleukin-5 | |
| 1 hr. 45 min. |
| 4-500 pg/mL | |
| Biotin | |
| Lavage Fluid, Serum, Supernatant | |
| ELISA | |
| 16191 | |
| Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) | |
| RUO | |
| 2°C to 8°C | |
| Mouse | |
| 24 hr. 30 min. |
For Research Use Only