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Invitrogen™ Oregon Green™ 488 BAPTA-1 dextran, Potassium Salt, 10,000 MW, Anionic
Dextran forms of these calcium indicators show dramatic reduction in both leakage and compartmentalization compared to AM ester forms
Brand: Invitrogen™ O6798
574.56 EUR valid until 2024-12-31
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Description
Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues.
- Cells may be physically loaded with cell-impermeant salt forms of these dextran-conjugated indicators using patch pipet or microinjection
- Fluorescence signal from these cells is measured using fluorescence microscopy
- Label (Ex/Em): Oregon Green 488 BAPTA-1 (494/523nm)
- Fluorescence intensity increase upon binding Ca2+: approximately 14 fold
- Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
- These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
- Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of problem
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling
Order Info
Shipping Condition: Room temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Potassium Assay | |
| Reagent | |
| Oregon Green™ | |
| Room Temperature |
| Fluorescence | |
| Fluorescence Microscope, Flow Cytometer, Microplate Reader | |
| Fluorescent Dye-Based | |
| 5 mg |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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