Invitrogen™ EnzChek™ Pyrophosphate Assay Kit

Description

The EnzChek Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically. The EnzChek Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters. The EnzChek Pyrophosphate Assay Kit is a modification of our EnzChek Phosphate Assay Kit. In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330nm to 360nm. The EnzChek Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.

• Sensitivity limit of 1 mM pyrophosphate (approx. 0.2μg/mL)
• Acommodates a pH range of 6.5 to 8.5

Anion Detection, Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Ionic Homeostasis and Signaling, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

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Shipping Condition: Room temperature