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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).
Marke: IBA Lifesciences 5-5168-001
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Beschreibung
pCSG-IBA mammalian expression vectors
- Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
- Stable cell lines can be selected by the neomycin resistance gene (NeoR).
- In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.
Spezifikation
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage | |
| Room temperature | |
| Direct Cloning | |
| Forward | |
| pCSG-IBA168 |
| Ampicillin | |
| Vector | |
| 12 months after shipping | |
| Mammalian | |
| Twin-Strep-tag-FLAG-tag | |
| 250 ng/μL | |
| 5 μg | |
| Episomal expression (oriP, EBNA-1) |
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