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Invitrogen™ LIVE/DEAD™ Fixable Red Dead Cell Stain Kit, for 488 nm excitation
The LIVE/DEAD™ Fixable Red Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Brand: Invitrogen™ L34971
This item is not returnable.
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Description
- Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
- Kit has been optimized and validated for use with a blue laser flow cytometer
- Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
- Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
- If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
- LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs
Optimal brightness
- The LIVE/DEAD™ Fixable Red stain was selected based on its fluorescent properties to provide a bright signal when excited with a yellow, green, or blue 488 nm laser
- The red-fluorescent reactive dye has an excitation maximum of ∼595 nm, making it ideal for use with the yellow laser, and an emission of ∼615 nm, allowing collection in the 610 or 630 BP filter
- LIVE/DEAD™ Fixable Red Dead Cell stain is also excited well with the blue 488 nm laser
- Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments
Specifications
| Impermeant | |
| Red | |
| LIVE/DEAD™ Fixable Red Dead Cell Stain Kit, for 488 nm excitation | |
| Viability Assay | |
| Stain | |
| 615 nm | |
| Solid | |
| LIVE/DEAD™ | |
| Room Temperature |
| Eukaryotic Cells | |
| Contains 2 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO. Store at -20°C. |
|
| Fluorescence | |
| Flow Cytometer | |
| LIVE/DEAD™ Fixable Red Dead Cell Stain | |
| 488, 595 nm | |
| Tube(s) | |
| 80 Assays | |
| DMSO (Dimethylsulfoxide) |
For Research Use Only. Not for use in diagnostic procedures.