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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red
Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Red/Blue is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.
Brand: Invitrogen™ R37610
250.04 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: 3 x 2.5mL NucBlue Live, 3 x 2.5mL propidium iodide
Description
ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set.
- NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
- Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617nm)
Suggestions for use
- NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
- In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Specifications
| Mammalian Cells, Eukaryotic Cells | |
| Store at 2° to 8°C, or at room temperature. | |
| Fluorescence | |
| Fluorescence Microscope | |
| 3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide | |
| 360, 535 nm | |
| ReadyProbes | |
| Room Temperature |
| Blue, Red | |
| ReadyProbes Cell Viability Imaging Kit, Blue/Red | |
| Viability Assay | |
| Cell Viability Imaging Kit | |
| 460, 617 nm | |
| Dropper bottles | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.