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Invitrogen™ Platinum™ SuperFi™ PCR Master Mix

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

329.00 EUR - 6090.00 EUR

Specifications

GC-Rich PCR Performance High
Polymerase Platinum SuperFi DNA Polymerase
Reaction Speed Fast
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
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Products 3
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
15525043
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Invitrogen™
12358010
100 Reactions
329.00 EUR
100 reactions
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15535043
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Invitrogen™
12358050
500 Reactions
1452.00 EUR
500 reactions
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Estimated Shipment: 10-04-2024
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15545043
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Invitrogen™
12358250
5 x 500 Reactions
6090.00 EUR
2500 reactions
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Estimated Shipment: 11-04-2024
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Description

Description

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.

Benefits of Platinum SuperFi PCR Master Mix include:
• Exceptional >300X Taq fidelity
• High specificity and increased yields with Platinum hot-start technology
• Robust amplification of difficult-to-amplify targets including those of sub-optimal purity or with ˃65% GC content
• Convenient workflow with room temperature reaction setup and 24 hr. bench top stability of pre-assembled reactions

Platinum SuperFi PCR Master Mix retains all the features of Platinum™ SuperFi™ DNA Polymerase, which is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.

Applications of Platinum SuperFi DNA Polymerase
• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

Using Platinum SuperFi PCR Master Mix
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use the Tm calculator on our website: www.thermofisher.com/tmcalculator.

Platinum SuperFi PCR Master Mix is supplied with a separate vial of SuperFi™ GC Enhancer designed for difficult and GC-rich templates (˃65% GC).

Platinum hot-start technology efficiently inhibits DNA polymerase activity at ambient temperatures, allowing convenient reaction setup at room temperature. In addition, pre-assembled PCR reactions may be stored at room temperature for up to 24 hr. prior to the PCR.

Specifications

Specifications

High
Fast
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
SuperMix or Master Mix
Platinum SuperFi DNA Polymerase
Wet or Dry Ice
300X
Blunt
20 kb or less
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SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.