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Invitrogen™ IL-10 Human ELISA Kit

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

718.00 EUR - 2235.00 EUR

Specifications

Accession Number P22301
Assay Range 15.36-600 pg/mL
Assay Sensitivity <3 pg/mL
Description Human IL-10 ELISA Kit
Conjugate Biotin
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Description

Description

Assay IL-10 (human interleukin-10) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-10 ELISA Kits.

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

P22301
<3 pg/mL
Biotin
ELISA Kit
No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
Colorimetric Microplate Reader
CSIF,GVHDS,IL-10A,TGIF,IL10
<10%
Absorbance at 450nm minus absorbance at 550nm
RUO
2°C to 8°C
1 hr. 20 min.
15.36-600 pg/mL
Human IL-10 ELISA Kit
Colorimetric ELISA (TMB substrate) with Plates
Plasma, Serum, Supernatant, Urine
Human
3586
<10%
HRP
Intra-assay CV <10%; inter-assay CV <10%
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL
Human
3 hr.
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