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  7. 11515674

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Brand:  Cytiva 11-0004-59

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Product Code. 11515674

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Description

Description

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 mL
7 mm
<4 mL/min.
3 to 12
4°C to 30°C
45 to 165 μm
Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
0.2 in.
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Polypropylene
6% Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
100 x 1 mL
Pre-packed Column
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  • Flammable liquid Category 3

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