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Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Brand:  Cytiva 17-5132-03


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Product Code. 11505025

  • 8900.00 EUR / 500mL
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Description

Description

  • Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
  • Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
  • Binding capacity and high flow properties allow scale-up
  • Mild elution conditions preserve protein antigenicity and function
  • Stable in all commonly used aqueous buffers
  • pH stability: 3 to 12

TRUSTED_SUSTAINABILITY
Specifications

Specifications

>10 mg/mL
<450 cm/hr.
Highly cross-linked 4% agarose
4% Highly Cross-linked Agarose
base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
500 mL
Glutathione and 10-carbon linker arm
Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
3 to 12
4°C to 30°C, 20% Ethanol
90 μm
Chromatography Media
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Safety and Handling

Safety and Handling

Product Identifier
  • Glutathione Sepharose 4 Fast Flow

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol

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