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Gibco™ DMEM, high glucose, pyruvate
GREENER_CHOICE

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

30.13 EUR - 716.00 EUR

Specifications

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
View More Specs

Requires: Supplementation


Products 5
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
13345364
GREENER_CHOICE
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Gibco™
11995065
500 mL
30.13 EUR
500mL
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13476146
GREENER_CHOICE
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Gibco™
11995073
10 x 500 mL
366.00 EUR
5 L
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17825170
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Gibco™
11995040
1000 mL
45.29 EUR
1000mL
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11350882
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Gibco™
11995115
5 L
366.00 EUR
Each
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11360882
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Gibco™
11995123
10 L
716.00 EUR
Each
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Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate

Without: HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
Sterile-filtered
No HEPES
Ambient
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Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 12 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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