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Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning

A ready-to-use, linear version of the popular cloning vector pUC19

Brand:  Invitrogen™ A13289

Product Code. 10343030

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Description

Description

The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.
  • Some of the key features and elements of the pUC19L vector include:
  • lacZα gene allows easy blue⁄white colony screening for selection of recombinants
  • pUC origin of replication (high copy vector)
  • Ampicillin resistance for selection in E. coli
  • 6 convenient and validated restriction sites flanking the cloning region
  • Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit

For Research Use Only. Not for use in diagnostic procedures.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store the product at -80°C. The vector is guaranteed stable for 6 months when properly stored.
PCR cloning, seamless cloning and genetic assembly
20
20 Reactions
Seamless Cloning Vector, Linear
Reporter: Beta-Gal (lacZ)
The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (A13288 - must be purchased separately).
GeneArt™ Linear pUC19L Vector for Seamless Cloning
13 kb total (vector plus all inserts), Up to 4 Fragments
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Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning > 20 Reactions

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