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Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning
A ready-to-use, linear version of the popular cloning vector pUC19
Brand: Invitrogen™ A13289
This item is not returnable.
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Description
The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.- Some of the key features and elements of the pUC19L vector include:
- lacZα gene allows easy blue⁄white colony screening for selection of recombinants
- pUC origin of replication (high copy vector)
- Ampicillin resistance for selection in E. coli
- 6 convenient and validated restriction sites flanking the cloning region
- Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Store the product at -80°C. The vector is guaranteed stable for 6 months when properly stored. | |
| PCR cloning, seamless cloning and genetic assembly | |
| 20 | |
| 20 Reactions | |
| Seamless Cloning Vector, Linear |
| Reporter: Beta-Gal (lacZ) | |
| The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (A13288 - must be purchased separately). | |
| GeneArt™ Linear pUC19L Vector for Seamless Cloning | |
| 13 kb total (vector plus all inserts), Up to 4 Fragments |