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Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Brand: Maxima K0252
557.00 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity - detects low copy number targets
- Wide linear range - accurate quantification across 9 orders of magnitude
- Reproducibility and convenience - ready-to-use 2X master mix
Specifications
| Contains: • 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions. • 250 μL ROX solution, 50 μM • 10 x 1.25 mL Nuclease-Free Water Store at -20°C. |
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| Tube | |
| qPCR | |
| Standard | |
| Maxima | |
| 1000 Reactions at 25 μL | |
| 2X | |
| DNA, dsDNA |
| SYBR | |
| High | |
| Taq DNA Polymerase | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| Real Time PCR SYBR Master Mix | |
| Gene Expression | |
| 1,000 Reactions | |
| 10 x 1.25 mL |
For Research Use Only. Not for use in diagnostic procedures.