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Molecular Probes™ Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Brand: Molecular Probes™ C10636

494.46 EUR valid until 2025-06-30
Use promo code "24074" to get your promotional price.

Product Code. 15342202

738.00 EUR / Each

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Description

The Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer. The Click-iT™ Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.

Multiplexable—compatible with R-PE (and tandems) and fluorescent proteins

Accurate—superior results compared to BrdU assays

Fast—results in as little as 60 minutes

View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.

Multiplexable
The Click-iT™ Plus formulation provides increased multiplexibility compared to the original Click-iT™ EdU Flow Cytometry assays. Click-iT™ Plus EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry, without loss of the accuracy or speed of the original Click-iT™ EdU assay.

An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT™ Plus EdU Flow Cytometry assay is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog that is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry, which is a copper-catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to the Alexa Fluor™ dye. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population.

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT™ Plus detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it can be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution, as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use Click-iT™ Plus EdU assay is compatible with cell cycle dyes. The Click-iT™ Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers, as well as conjugates labeled with standard fluorophores including R-PE, R-PE tandems, and fluorescent proteins (GFP and mCherry).

Quick and Simple Protocol
The Click-iT™ Plus EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling. However, EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:
1. Treat cells with EdU.
2. Fix and permeabilize cells.
3. Detect S-phase cells with Click-iT™ Plus detection cocktail for 30 min.
4. Wash once.
5. Analyze.
Results can be seen in as little as 60 minutes in some circumstances, but we recommend 90 minutes for all applications.

Specifications

Content And Storage	Contains EdU (5-ethynyl-2' -deoxyuridine), Pacific Blue™ picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ fixative, Click-iT™ saponin-based permeabilization and wash buffer, Copper Protectant, and Click-iT™ EdU buffer additive. Store at 2°C to 8°C Dessicate and protect from light.
For Use With (Application)	Flow Cytometry
Product Type	Flow Cytometry Assay Kit
Emission	404⁄450
Product Line	Click-iT™
Shipping Condition	Room Temperature

Detection Method	Fluorescence
For Use With (Equipment)	Flow Cytometer
Dye Type	Pacific Blue™
Format	Tube(s)
Quantity	50 assays

Product Suggestions Documents

Documents

Product Information

Manuals

User Guide: Click-iT Plus EdU Flow Cytometry Assay Kits Version: E.0 File size: 501.2 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2845254	Certificate TypeCertificate of Analysis	Date18/06/2024	Catalog NumberC10636
Lot Number2790090	Certificate TypeCertificate of Analysis	Date22/11/2023	Catalog NumberC10636
Lot Number2575946	Certificate TypeCertificate of Analysis	Date03/04/2023	Catalog NumberC10636
Lot Number2521020	Certificate TypeCertificate of Analysis	Date16/08/2022	Catalog NumberC10636
Lot Number2505973	Certificate TypeCertificate of Analysis	Date08/06/2022	Catalog NumberC10636

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