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Molecular Probes™ CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells.

141.00 EUR - 473.00 EUR

Specifications

Format Kit
Product Type Caspase Assay Kit
Content And Storage Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
Flow Cytometer Laser Lines 488
Shipping Condition Wet Ice
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Product Code Brand Quantity Price Quantity & Availability  
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100 Assays
473.00 EUR
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15583329
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Molecular Probes™
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20 Assays
141.00 EUR
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Description

Description

The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

Specifications

Specifications

Kit
Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
Wet Ice
Fluorescence
Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Other Label(s) or Dye(s)
Caspase Assay Kit
488
CellEvent™
Flow Cytometry
CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
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Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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