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BD Ac-DEVD-CHO Inhibitor
To identify and quantify Caspase-3 activity in apoptotic cells, and to study events downstream of Caspase-3 activation
Brand: BD 556465
Requires : Apoptotic and non-apoptotic cells, phosphate buffered saline (PBS) Wash Buffer: 140mM NaCl, 2.7mM KCl, 10mM KH2PO4 dissolved in distilled, autoclaved water. Adjust the pH to 7.4 using hydrochloric acid, Cell Lysis Buffer: 10mM Tris (pH 7.5), 130mM NaCl, 1% Triton -X-100, 10mM NaPi, 10mM NaPPi, Caspase-3 (CPP32), Ac-DEVD-CHO Inhibitor, and substrate Ac-DEVD-AMC, Protease Assay Buffer: 20mM HEPES (pH 7.5), 10% glycerol, 2mM DTT
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Description
Members of ICE/CED-3 cysteine protease family have key roles in inflammation and mammalian apoptosis. The ICE family member Caspase-3 (also known as CPP32, Yama, apopain) is activated early in apoptosis and appears to be involved in proteolysis of several important molecules, including poly (ADP ribose) polymerase (PARP)
- Synthetic tetrapeptide inhibitor for Caspase-3
- Used in protease assays
- Cleaves PARP from 116kD to 85kD residual fragment
- Cleavage site in PARP is C-terminal to Asp-216
- Upstream sequence of cleavage site, DEVD (Asp-Glu-Val-Asp), is utilized as basis for highly specific Caspase-3 substrate, Ac-DEVD-AMC and Caspase-3 inhibitor,Ac-DEVD-CHO
- Storage Buffer: Lyophilized powder
- Format: Purified
Functional assay
Specifications
| Functional assay | |
| -20°C | |
| 502.5 | |
| Purified |
| Lyophilized powder | |
| 1 mg | |
| Ac-DEVD-CHO |
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