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  7. Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
  8. 10117254

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Brand:  Applied Biosystems™ N8080234

523.60 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 10117254

  • 748.00 EUR / Each

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Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
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Description

Description

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

TRUSTED_SUSTAINABILITY
Spécification

Spécification

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –15 to –25°C.
Reverse Transcription
200 reactions
Yes
Real Time PCR (qPCR)
First-Strand cDNA
Separate components
Up to 5 kb
Kit
42°C
MultiScribe
Dry Ice
50 U/μL
200 Reactions
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.