missing translation for 'onlineSavingsMsg'
Learn More

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

287.00 EUR - 17090.00 EUR

Specifications

GC-Rich PCR Performance Low
Polymerase AmpliTaq Gold DNA Polymerase
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Concentration 5 U/μL
View More Specs

Products 6
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10490735
View Documents
Applied Biosystems™
4311806
250 Units
287.00 EUR
Each
Please to purchase this item. Need a web account? Register with us today!
10685095
View Documents
Applied Biosystems™
4311816
1000 Units
994.00 EUR
1000 units
Please to purchase this item. Need a web account? Register with us today!
10156624
View Documents
Applied Biosystems™
4311814
1500 Units This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
10428444
View Documents
Applied Biosystems™
4311820
3000 Units
2580.00 EUR
3000 units
Please to purchase this item. Need a web account? Register with us today!
10360545
View Documents Promotion Details
Applied Biosystems™
4311818
5000 Units
3870.00 EUR
5000 units
Please to purchase this item. Need a web account? Register with us today!
10640796
View Documents
Applied Biosystems™
4317742
25,000 Units
17090.00 EUR
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Features

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Gold Buffer with MgCl2

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.

GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

Notes

  • AmpliTaq Gold 360 DNA Polymerase is available for even better PCR performance.
  • AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Specifications

Specifications

Low
5' - 3'
5 U/μL
Built-In Hot Start
Separate Components
AmpliTaq Gold DNA Polymerase
DNA Polymerase
1X
3'-A
5 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.