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Molecular Probes™ alamarBlue™ Cell Viability Reagent
AlamarBlue™ Cell Viability Reagent
Brand: Molecular Probes™ DAL1100
481.80 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
The alamarBlue™ dye is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.Applications:
The alamarBlue™ dye as a cell viability assay reagent
The alamarBlue™ assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.
A safe, convenient, and versatile assay
AlamarBlue™ dye is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue™ dye is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. AlamarBlue™ dye is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue™ indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.
Experimental detection of alamarBlue™ dye
Specifications
| Bacteria, Eukaryotic Cells, Fungal Cells | |
| Store in refrigerator at 2°C to 8°C. |
|
| Colorimetric, Fluorescence | |
| Spectrophotometer, Microplate Reader | |
| Resazurin | |
| 530-560 nm | |
| 96-well plate, Cuvettes, 384-well plate | |
| alamarBlue™ | |
| Wet Ice |
| Blue | |
| alamarBlue™ Cell Viability Reagent | |
| Viability Assay | |
| Cell Viability Reagent | |
| 590 nm | |
| Liquid | |
| 1 to 4 hr. (Up to 24 hr. for low cell numbers) | |
| 100 mL | |
| High-throughput Compatible |
For Research Use Only. Not for use in diagnostic procedures.