missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Western Blot Substrates and Substrate Kits
  5. 10066433

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Brand:  Thermo Scientific™ 34018


Product Code. 10066433

  • 191.00 EUR / 250mL

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

  • HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

  • Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

  • Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

  • Complete – does not require other buffers or addition of hydrogen peroxide

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Substrate
Liquid
250 mL
Horseradish Peroxidase
Colorimetric
1-Step™
Horseradish Peroxidase
Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • 1-Step TMB-Blotting, 250 mL

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • Specific target organ toxicity Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H371-May cause damage to organs.

Precautionary Statement
  • P270-Do not eat, drink or smoke when using this product.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.

Supplemental information
  • MIXTURE LIST-Contains : Methyl alcohol

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.