Applied Biosystems™ StepOne™ & StepOnePlus™ Real-Time PCR Systems

banner_promo_stepone_steponeplus
StepOne

The StepOne™ (48 well) and StepOnePlus™ (96 well) Real-Time PCR Systems are remarkably simple to use. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced real-time PCR researchers. Despite their simplicity, the advanced technology will deliver you precise and reliable results every time.

New Lower Price!

We’ve just significantly reduced the list prices of both the StepOne and StepOnePlus Real-Time PCR Systems, so you can benefit from great features for even less. High-performance real-time PCR is now more affordable than ever.

All you need for high performance real-time PCR

  • Plug in and go – system is delivered ready to use
  • Perform fast PCR reactions in less than 40 minutes
  • Intuitive data analysis and instrument control software that performs for a wide array of genomic assays
  • LCD touchscreen and USB drive provide configuration flexibility and enable PC-free operation
  • Remote monitoring and email notification for convenience and time-savings
  • Choice of 48 or 96 well
  • Sensitive and long-life three or four colour optical LED recording system
  • VeriFlex™ Block technology for enhanced PCR functionality and precise temperature control (StepOnePlus™ only)
  • Easy to upgrade a StepOne™ to a StepOnePlus™ System – flexible to suit your changing requirements
  • Ultra-compact footprint to fit any lab

StepOne™
StepOne™ System StepOnePlus™ System
Throughput/Wells 48 96
FAM™/SYBR® Green dyes
VIC®/JOE™ dyes
NED™ dye
NED/TAMRA™ dyes
VeriFlex™ Block
    Catalog No. Description Price Stock Quantity  
success
15331295 Details
GREENER_CHOICE
 		StepOnePlus 96well Real-Time PCR System with Tower 29230.00 EUR
Estimated Shipment: 16-04-2025
to see stock.
TRUSTED_SUSTAINABILITY
Please to purchase this item. Need a web account? Register with us today!
  
Product Specifications
Thermal Uniformity ± 0.50°C (35°C to 95°C) of Setpoint/Display Temperature
Optics 4 Emission Filters, Blue LED Excitation source, photodiode
Format 96-well Plate, 8-tube Strips, 0.1 mL Tubes
High-throughput Compatibility Multiplexing
Content And Storage Desktop Computer
External Computer Minitower
Run Time <2 hrs/run (Standard Mode), <40 min/run (Fast Mode)
Peak Block Ramp Rate 4.6°C/s
Product Line StepOnePlus™
Weight 24 kg (52 lb.)
Reaction Volume Range 10–30 μL (Standard Curve Experiments: 40 μL in Standard Mode is Validated)
Dynamic Range Linear Dynamic Range greater than 9 Log Units (Detection)
Passive Reference Dye ROX (Separate Tube), No ROX, ROX (Pre-mixed)
For Use With (Equipment) StepOnePlus™
Sensitivity 1 Copy
Quantity 1 system
Detection Method SYBR, Primer-Probe Detection
Thermal Cycling System Peltier-based System
Temperature Range (Metric) 4 to 100°C
Sample Ramp Rate Fast Mode: ± 2.2°C/s, Standard mode: ± 1.6°C/s
Display Type LCD Touchscreen
Reaction Speed Fast, Standard
Dimensions 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
Thermal Accuracy ± 0.25°C (35°C to 95°C) of Display Temperature
Capacity 96 x 0.1 mL Tubes, 12 x 8-tube Strips, 1 x 96-well Plate
Calibrated Dye VIC™, SYBR™ Green I, ROX™, JOE™, NED, FAM™, TAMRA™
15351295 Details
StepOnePlus Real-Time PCR System Upgrade 10880.00 EUR
Estimated Shipment: 16-04-2025
to see stock.
TRUSTED_SUSTAINABILITY
Please to purchase this item. Need a web account? Register with us today!
  
12170012 Details
GREENER_CHOICE
 		StepOne Real-Time PCR System, Tower 19450.00 EUR
Estimated Shipment: 16-04-2025
to see stock.
TRUSTED_SUSTAINABILITY
Please to purchase this item. Need a web account? Register with us today!
  
Product Specifications
Thermal Uniformity ± 0.50°C (35°C to 95°C) of Setpoint/Display Temperature
Sensitivity 1 Copy
Height (Metric) 51.2 cm
Description Scan Time: <2 hr./run (Standard Mode), <40 min./run (Fast Mode)
Quantity 1 Instrument
For Use With (Application) Genotyping and Genomic Profiling, Real Time PCR (qPCR), Real Time PCR-based Gene Expression Profiling
Content And Storage Desktop Computer
Block Configurations 0.1 mL Tubes, 48-well Plate
Pre-Calibrated Dyes FAM™, JOE™, ROX™, SYBR™ Green I, VIC™
Max. Heating Rate (Thermal Block) 4.6°C/s
Volume (Metric) Thermal Block Sample 10 to 30μL (Standard Curve Experiments: 40μL in Standard Mode is Validated)
Thermal Range 4°C to 100°C
Weight 24 kg (52 lb.)
Detection 3 Emission Filters, LED, Photodiode
Voltage 120 V
Multiplex Multiplexing
Software Analysis Modes Fast mode: ±2.2°C/s, Standard mode: ±1.6°C/s
Depth (Metric) 42.7 cm
Thermal Accuracy ± 0.25°C (35°C to 95°C) of Display Temperature
Dynamic Range Linear Dynamic Range greater than 9 Log Units (Detection)
15320855 Details
GREENER_CHOICE
 		StepOnePlus Real-Time PCR System, Laptop 29090.00 EUR
Estimated Shipment: 15-05-2025
to see stock.
TRUSTED_SUSTAINABILITY
Please to purchase this item. Need a web account? Register with us today!
  
Product Specifications
Thermal Uniformity ± 0.50°C (35°C to 95°C) of Setpoint/Display Temperature
Optics 4 Emission Filters, Blue LED Excitation source, photodiode
Format 96-well Plate, 8-tube Strips, 0.1 mL Tubes
High-throughput Compatibility Multiplexing
Content And Storage Laptop Computer
External Computer Notebook
Run Time <2 hrs/run (Standard Mode), <40 min/run (Fast Mode)
Peak Block Ramp Rate 4.6°C/s
Product Line StepOnePlus™
Weight 24 kg (52 lb.)
Reaction Volume Range 10–30 μL (Standard Curve Experiments: 40 μL in Standard Mode is Validated)
Dynamic Range Linear Dynamic Range greater than 9 Log Units (Detection)
Passive Reference Dye ROX (Separate Tube), No ROX, ROX (Pre-mixed)
For Use With (Equipment) StepOnePlus™
Sensitivity 1 Copy
Quantity 1 system
Detection Method SYBR, Primer-Probe Detection
Thermal Cycling System Peltier-based System
Temperature Range (Metric) 4 to 100°C
Sample Ramp Rate Fast Mode: ± 2.2°C/s, Standard mode: ± 1.6°C/s
Display Type LCD Touchscreen
Reaction Speed Fast, Standard
Dimensions 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
Thermal Accuracy ± 0.25°C (35°C to 95°C) of Display Temperature
Capacity 96 x 0.1 mL Tubes, 12 x 8-tube Strips, 1 x 96-well Plate
Calibrated Dye VIC™, SYBR™ Green I, ROX™, JOE™, NED, FAM™, TAMRA™
12160012 Details
GREENER_CHOICE
 		StepOne Real-Time PCR System, Laptop 19260.00 EUR
Estimated Shipment: 16-04-2025
to see stock.
TRUSTED_SUSTAINABILITY
Please to purchase this item. Need a web account? Register with us today!
  
Product Specifications
Thermal Uniformity ± 0.50°C (35°C to 95°C) of Setpoint/Display Temperature
Optics LED, 3 Emission Filters, Photodiode
Format 48-well Plate, 0.1 mL Tubes
High-throughput Compatibility Multiplexing
Content And Storage Laptop Computer
External Computer Notebook
Run Time <2 hrs/run (Standard Mode), <40 min/run (Fast Mode)
Peak Block Ramp Rate 4.6°C/s
Product Line StepOne™
Weight 23.6 kg (52 lb.)
Reaction Volume Range 10–30 μL (Standard Curve Experiments: 40 μL in Standard Mode is Validated)
Dynamic Range Linear Dynamic Range greater than 9 Log Units (Detection)
Passive Reference Dye ROX (Separate Tube), No ROX, ROX (Pre-mixed)
For Use With (Equipment) StepOne™
Sensitivity 1 Copy
Quantity 1 instrument
Detection Method SYBR, Primer-Probe Detection
Thermal Cycling System Peltier-based System
Temperature Range (Metric) 4 to 100°C
Sample Ramp Rate Fast Mode: ± 2.2°C/s, Standard mode: ± 1.6°C/s
Display Type LCD Touchscreen
Reaction Speed Fast, Standard
Dimensions 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
Thermal Accuracy ± 0.25°C (35°C to 95°C) of Display Temperature
Capacity 48 x 0.1 mL Tubes, 1 x 48-well Plate
Calibrated Dye VIC™, SYBR™ Green I, JOE™, FAM™, ROX™
Resources

Demo

This field is required