Thermo Scientific™ Giolitti-Cantoni Broth (Dehydrated)

Description

Selectively enrich Staphylococcus aureus from food and feedstuffs when following ISO 6888-3:2003 with Thermo Scientific™ Oxoid™ Giolitti-Cantoni Broth (Dehydrated) when used with sterile solution of Potassium tellurite (SR0030). Mannitol and sodium pyruvate in the medium act as growth stimulants for staphylococci and aid detection of the organisms when present in small numbers¹.

The growth of Gram-negative lactose-fermenting bacilli is inhibited by lithium chloride and Gram-positive bacilli are inhibited by potassium tellurite in combination with glycine.

Giolitti-Cantoni Broth is an anaerobic enrichment broth for Staphylococcus aureus in food samples.

• Selective: lithium chloride inhibits Gram-negative bacilli, while potassium tellurite, in combination with glycine, inhibits Gram-positive organisms other than staphylococci.
• Easy-to-read: the presence of coagulase-positive staphylococci is indicated by the reduction of tellurite, resulting in a blackening of the broth or a black precipitate.

Giolitti-Cantoni Broth is recommended for the detection of Staphylococcus aureus in dried baby milk and other weaning foods where the organism should be absent from 1g of test material².

The medium is suitable for the examination of meat and meat products³. For this purpose, the concentration of the potassium tellurite must be reduced to 0.35% and it is recommended that the weight of the test sample should be reduced to 0.1-0.01g.

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