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Macherey-Nagel™ NucleoSpin™ Plasmid Columns
Replacement columns for use with NucleoSpin™ Plasmid kits for rapid, small-scale preparation of high-purity, sequencing-grade plasmid DNA.
Specifications
| Color | Clear |
|---|---|
| Format | Mini Spin Column |
| Isolation Technology | Silica Membrane Technology |
| Sample Type | E. coli Culture |
| Efficiency | Binding capacity: 50 μg |
Description
NucleoSpin™ Plasmid
- For rapid small scale preparation high-purity plasmid DNA
- Fast procedure – 6 Mini preps in 25 min
- High capacity – up to 50 μg of plasmid DNA
- Chaotropic salt wash buffer – extra pure DNA
- Downstream applications: cloning, sequencing, PCR, transformation, restriction analysis
- High-copy plasmids from E. coli
- Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
- Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
- Supplementary protocol for M13 DNA availabl
Specifications
| Clear | |
| Silica Membrane Technology | |
| Binding capacity: 50 μg | |
| Sequencing-grade plasmid DNA | |
| Preparation of high-purity plasmid DNA | |
| NucleoSpin Plasmid lidded Column |
| Mini Spin Column | |
| E. coli Culture | |
| Elution volume: 50 μL | |
| NucleoSpin™ Plasmid Kit | |
| Columns only | |
| 2 - 10 mL |
Safety and Handling
Product Identifier
Supplemental information
- NucleoSpin Plasmid
Supplemental information
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/2-pr