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Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 594 conjugate
Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry
Marca: Invitrogen™ Z23374
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Descripción
- Fluorescence of fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, fluorescence of the Alexa Fluor, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10
- Label (Ex/Em): Alexa Fluor594 (approx. 590/617nm)
- Particle: Zymosan (S. cerevisiae)
- Provided as lyophilized powders
- Approximately 3 x 108 E. coli or S. aureus particles/mg solid and approximately 2 x 107 zymosan particles/mg solid
- Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
- Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
- Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
- Sall particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection
Información de órdenes
Shipping Condition: Room Temperature
Especificaciones
| Store in freezer (-5 to -30°C) and protect from light. | |
| Fluorescence Microscope | |
| Alexa Fluor™ 594 | |
| 3 to 10 | |
| 2 mg | |
| S. cerevisiae |
| Fluorescence | |
| Supplement | |
| Lyophilized Powder | |
| Alexa Fluor™, BioParticles™ | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.