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Invitrogen™ EnzChek™ Phosphatase Assay Kit
Continuous assay of prostatic acid phosphatase and protein phosphatase 1
Brand: Invitrogen™ E12020
This item is not returnable.
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Description
Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Order Info
Shipping Condition: Room temperature
Specifications
| Blue | |
| Fluorescence | |
| Microplate Reader | |
| DiFMU | |
| EnzChek™ | |
| Room Temperature | |
| Chemical Substrate | |
| Phosphatases |
| Store in freezer -5°C to -30°C and protect from light. | |
| Phosphatase Assay | |
| Phosphatase Assay | |
| 360⁄460 | |
| 1000 Assays | |
| DiFMUP | |
| Phosphatase Substrate |
For Research Use Only. Not for use in diagnostic procedures.