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Invitrogen™ ICAM-1 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMICAM1ALPHAX10

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Product Code. 18587874

  • 4815.00 EUR / Pack of 10

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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
This item is not returnable. View return policy

Description

Description

The Mouse Intercellular Adhesion Molecule 1 (Ms ICAM-1) ELISA quantitates Ms ICAM-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ICAM-1. Principle of the method The Mouse ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

P13597
20 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Serum
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
15894
<12%
Adhesive Plate Covers
10 x 96 Tests
Inflammation
2°C to 8°C
Mouse
4 hr. 45 min.
24.69 to 6000 pg/mL
20 pg/mL to 6000 pg/mL
Biotin
Other Proteins
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP- 1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF.
Mouse
Colorimetric Microplate Reader
CD54,Icam-1,Ly-47,MALA-2
<10%
HRP
RUO
Plasma, 2 µL; Serum, 2 µL; Supernatant, 100 µL
CD54, Icam-1, Ly-47, MALA-2
1 hr. 20 min.
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