Rat IgG2a Isotype Control, PE, Invitrogen™

Description

PA5-33216 has been shown to be negative on all mouse cells and cell lines tested. This antibody recognizes a human cell surface marker, and therefore cannot be used as a negative control in this species. Test results have shown that PA5-33216 is also suitable for use as a negative control with canine tissues. Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4oC in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.