IBA GmbH

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Products

IBA Lifesciences™ Magnetic Separator

Aids fast purification of Strep-tag™ proteins. Magnetic Separator for 24 rxntubes, 1piece

IBA Solutions For Life Science™ ApU-dinucleotide IBA

1 MMOL ApU-dinucleotide 1µmol Store in Freezer at

IBA Solutions For Life Science™ GpC-dinucleotide IBA

5 MG GpC-dinucleotide 5mg Store in Freezer at -20C

IBA Solutions For Life Science StarGate™ pLSG-IBA Acceptor Vectors for Baculo

Transfer vectors that allow for high-level expression in insect cells by introducing the gene of interest into the Polyhedrin gene locus of AcMNPV DNA by homologous recombination. pLSG-IBA33; 5µg

IBA Solutions For Life Science Cyanine 5-ApG-PH Transcription Starter

A DNA/RNA dinucleotide (oligonucleotide for 5´-labeled transcripts) used by RNA polymerases as an initiator for RNA synthesis and therefore very useful for transcription analysis. Cyanine 5-ApG-PH

IBA GmbH CD31 Isolation Kit for FABian™

For automated positive cell separation. The Isolation Kit for FABian&trade provides label-free target cells without the use of high affinity antibodies and magnetic beads CD31 Isolation Kits for FABian(R), human, 10 Kits

IBA Solutions For Life Science StarGate™ pESG-IBA Acceptor Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen. pESG-IBA105; 5µg

IBA GmbH™ Small Mammalian Expression Vector

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA GmbH™ Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system. pDSG-IBAwt2; 5µg

IBA Solutions For Life Science™ 2'-Amino-2'-dUTP IBA

500 NMOL 2'-Amino-2'-dUTP 0.5µmol Store in Freezer

IBA Solutions For Life Science 6-Chloro-7-deaza-GTP

A modified nucleoside-triphosphate (modified 2´-ribonucleoside-5´-triphosphates) that helps in studying RNA-protein interactions. 6-Chloro-7-deaza-GTP

IBA GmbH™ Strep-Tactin™XT Superflow™ Cartridge

IBA GmbH™ Strep-Tactin™XT Superflow™ Cartridge is a reliable one-step purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins with high affinity and unsurpassed purity. Strep-Tactin Superflow XT 1ml cartridge (with 10-32 connection for HPLC and Äkta)

IBA Solutions for Life Science™ Strep-tag™ Washing Buffer, 10X

100 ML Strep-tag(R) washing buffer (10x buffer W)

IBA Solutions For Life Science™ 2-Diaminopurine-2'-desoxyriboside-TP IBA

500 NMOL 2,6-Diaminopurine-2'-desoxyrib oside-TP

IBA Solutions For Life Science™ ApA-dinucleotide IBA

5 MG ApA-dinucleotide 5mg Store in Freezer at -20C

IBA Lifesciences™ Microtiter Plate

Strep-Tactin® coatedmicroplate, 1 plate, 1plate

IBA Solutions For Life Science C8-Alkyne-dCTP

A modified nucleoside-triphosphate (3'-modified oligos) that is suitable for subsequent modifications with fluorescent or non-fluorescent Azides. C8-Alkyne-dCTP

IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter. pYSG-IBAwt1; 5µg

IBA Solutions For Life Science™ N6-Methyl-2'-dATP IBA

500 NMOL N6-Methyl-2'-dATP 0.5µmol Store in Freeze

IBA Solutions For Life Science™ UpU-dinucleotide IBA

1 MMOL UpU-dinucleotide 1µmol Store in Freezer at

IBA GmbH™ MHC I-Strep HLA-A*0201 CMV IE-1 VLEETSVML

IBA GmbH™ MHC I-Strep HLA-A*0201 CMV IE-1 VLEETSVML is for fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells. MHC I-Strep HLA-A*0201, CMV IE-1, VLEETSVML; 40 µl

IBA GmbH™ Mammalian Expression Vector with No Secretion

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA GmbH™ Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR). pCSG-IBA143; 5µg

IBA GmbH™ E. coli Expression Vector with No Secretion

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA GmbH™ E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli. pASG-IBA143; 5µg

IBA Solutions For Life Science StarGate™ Downstream Fusion Vector pCFUSE-IBA-11

For downstream positioning of an inserted gene of interest in the final Fusion Donor vector when linking two genes cloned in separate Donor Vectors using the StarGate™ transfer cloning system. pCFUSE-IBA-11; 5µg

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension can be used to pack your own columns for protein purification. 50ml Strep-Tactin XT Superflow 50% suspension

IBA GmbH™ Streptamer™ CD3/CD28 Premix for T Cell Expansion

Streptamer™ CD3/CD28 Premix for T Cell Expansion is for reversible CD3 and CD28 receptor specific cell activation/expansion using the Streptamer™ technology. Streptamer®CD3/CD28 premix for T cell expansion for 1.6 x 10¬7 cells; 480 µl

IBA Solutions For Life Science™ 4-Thio-UTP

500 NMOL 4-Thio-UTP 0.5µmol Store in Freezer at-20°C

IBA Solutions For Life Science™ UpC-dinucleotide IBA

10 MG UpC-dinucleotide 10mg Store in Freezer at

IBA Lifesciences StrepMAB-Immo DY-649 Conjugate

IBA Lifesciences Protein detection with Strep-tag™ antibodies designed for the detection of Strep-tag™ II and Twin-Strep-tag™ in Immunofluorescence, and FACS. 50UG StrepMAB-Immo DY-649 conjugate

IBA Solutions For Life Science 2'-Fluoro-2'-dUTP

A modified nucleoside-triphosphate (modified oligos) that prevents nuclease degradation of RNAs. 2'-Fluoro-2'-dUTP

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Featured Products

Twin-Strep-tag™/Strep-Tactin™XT


Twin-Strep-tag

The purification system with the highest affinity – one tag for expression, purification, detection and immobilization

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Immobilization with near covalent binding affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR experiments »

  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated

High affinity meets high capacity!


High affinity meets high capacity!

Strep-Tactin™XT Superflow™ high capacity »

  • Higher protein yields compared to all other Strep-Tactin™ resins
  • Dynamic binding capacity of ~15 mg protein per ml resin
  • Elution of concentrated protein due to small elution volumes
  • Cost efficent: small price for more protein

Try our new high affinity Strep-tag™ system


Try our new high affinity Strep-tag® system

Starter Kit consists of:

  • Strep-Tactin™XT Superflow™ gravity flow column
  • Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
  • Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
  • Strep-Tactin™ conjugated with HRP (for Western blot detection)
  • Short protocol
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation

Fab-TACS™ Gravity and FABian™ »

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™ »

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™ »

  • Completely reversible reagents
  • Detachment from the cells at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Non-magnetic and bead-free

Peptides for T cell stimulation »

  • Purity > 80 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ »

Detection of receptor-specific T cells with Fab Streptamers™ »

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning & Transfection


Cloning & Transfection

More than 150 expression vectors »

  • Variety of affinity tags
  • Expression hosts:  E. coli, mammalia, insect cells, yeast
  • StarGate cloning system

Mammalian Expression


The MEXi system »

Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.

Protein Purification


Protein Purification

Strep-tag™ system from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA »

  • Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
  • Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated

Immunoprecipitation


Selector resins based on high-affinity single-domain antibodies (sdAb)

  • High capacity
  • Minimal unspecific binding
Oligos
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DNA


RNA


DNA & RNA

Standard DNA oligonucleotides

  • Long range up to 200 bases*
  • Large scale oligonucleotides
  • 3’-, 5’- or internally modified

Random RNA, dsRNA, siRNA, miRNA

  • Long range up to 100 bases*
  • 3’-, 5’- or internally modified
  • 2‘-modified: -O-Me, -F, -NH2
  • Nucleoside Triphosphates
  • RNA and RNA/DNA hybrid
  • Dinucleotides

*depending on sequence and scale

Fluorescence Labels


Non-Fluorescence Labels


Fluorescence Labels

E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami

  • For dual and multiply labeled DNA and RNA
  • Excellent high quality 
  • More than 200 labels to choose from
  • Quenchers such as BBQ and BHQ™
  • Completely new dye combinations available

For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more

  • More than 80 internal, 5’- and 3’- DNA and RNA modifications 

Backbone Modifications


Aptamers


Backbone Modifications
  • Phosphorothioates

Custom synthesis of DNA and RNA aptamers

  • High affinity 
  • Low immunogenicity
Resources Strep-Tactin®XT Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.