Protein Extraction and Purification

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 10/300 GL

Thermo Scientific™ Halt™ Phosphatase Inhibitor Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors. X24 HALT(TM) PHOSPHATASE INHIBITOR SINGLE-USE

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 200ML Sodium Dodecyl Sulfate (SDS), 20% Solution (Electrophoresis), (as Fatty Alcohol Sulfate):

SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 200ML Sodium Dodecyl Sulfate (SDS) 10% Solution (Electrophoresis, DNase RNase & protease free)

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 500ML Triton X-100 for Electrophoresis

Invitrogen™ eBioscience™ RBC Lysis Buffer, 1x

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood. eBioscience 1X RBC LysisBuffer 200 ml

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds. Bestatin, 10 mg

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. HALT PROTEASE & PHOSPHATASE SINGLE-USE INHIBITORCocktail, EDTA free, 24 x 100µl

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators. PIERCE ANTI-HA MAGNETIC BEADS, 5 ML

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 MEDIUM 100 GRS

GE Healthcare Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.  GE Healthcare Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 5/150 GL

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation. 1 ML PIERCE STREPTAVIDIN MAGNETIC BEADS 1ML STOREin Fridge at 4C

GE Healthcare SpeedBeads™ Magnetic Neutravidin Coated Particles, 1 um, 1% solids, 0.05% Na azide

Capture of Biotinylated Probes. Their “cauliflower-like” structure gives Neutravidin beads high binding capacity. Neutravidin SpeedBeads combine fast reaction kinetics and low, non-specific binding for increased throughput and precision in immunoassay and molecular biology applications. 100mL SpeedBead Magnetic Neutravavidin Coated particles,DSMG-NA, 1 um, 1% solids

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEA INHIB SINGLE-US 2400µLEDTA-Free, 24 x 100µl

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. MEM-PER PLUS KIT, GOOD FOR 50 REACTIONS, 1 KIT

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose. X25 HIGH CAPACITY ENDOTOXIN 0,5ML

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit. 15ML NUCLEAR AND CYTOPLASMIC EXTRACTION REAGENT,

Thermo Scientific™ Triton X-100 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

1000 ML TRITON X-100 SURFACT-AMPS DETERGENT SOLUTIon 1000mL Store at Room temperature

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation. COMBINED INH EDTA-FREE SAMPLE 1 VIAL

GE Healthcare Tricorn Columns

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE. EMPTY COLUMN TRICORN 10/50 CHROMATOGRAPHY

Invitrogen™ Total Exosome Isolation Reagent (from cell culture media)

Easily isolate exosomes and avoid time-consuming ultra-centrifugation Total Exosome Isolation (from Cell Culture Media),50 ml

Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions. B-PER REAG(IN PHOSPHATE BUFF)500ML

GE Healthcare Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency. SEPHADEX G-50 SUPERFINE 100 G

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents. CELL SURFACE PROT ISOLATION KITStore in Fridge at 4°C

GE Healthcare Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. GE Healthcare Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species. SEPHADEX LH-20, 25 GRS

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